C0979
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 | Immunoblotting Human HepG2 cell extract was separated on SDS-PAGE and probed with Monoclonal PA to Catalase CL:CAT-505 (PREPK) (Cat. No. C0979). The antibody was developed with AlkPhos APA Mouse Fab ads HIgG (Cat. No. A1293) and a NBT/BCIP substrate. Antibody concentration 0.5-1 μg/mL |
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C0979
Sigma
Monoclonal Anti-Catalase antibody produced in mouse
~2 mg/mL, clone CAT-505, purified immunoglobulin, buffered aqueous solution
| MDL number: | MFCD00802760 |
Description
| Immunogen | human erythrocyte catalase |
| Preparation Note | Purified from culture supernatant of hybridoma cells grown in a bioreactor. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH7.4, containing 15 mM sodium azide |
Properties
| antibody form | purified immunoglobulin |
| clone | CAT-505 (monoclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 60 kDa |
| species reactivity | human, mouse, bovine, rat |
| concentration | ~2 mg/mL |
| application(s) | immunoblotting: 0.5-1 μg/mL using a whole extract of HepG2 cells |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| isotype | IgG1 |
| Gene Information | human ... CAT(847) |
| rat ... Cat(24248) | |
| mouse ... Cat(12359) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
Related Products
| Array | XP725, Panorama® Antibody Microarray - XPRESS Profiler725 Kit |
References
| reference | McGrath, A.M., et al., Alterations in the Rat Serum Proteome During Liver Injury from Acetaminophen Exposure J. Pharmacol. Exp. Ther. 318, 792-802, (2006) Abstract |
| Guy, J., and Hauswirth, W.W., Dual gene therapy with extracellular superoxide dismutase and catalase attenuates experimental optic neuritis Mol. Vis. 13, 1-11, (2007) Abstract |
